Western Blot Positive WB detected in:Mouse liver tissue All lanes:Di-methyl-Histone H3.1(K9)antibody at 0.9μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 KDa Observed band size: 15 KDa
Immunofluorescence staining of Hela cells with CSB-RA010418A09me2HU at 1:56,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
It differs from different batches. Please contact us to confirm it.
保存緩沖液:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式:
Liquid
應(yīng)用范圍:
ELISA, WB, IF
推薦稀釋比:
Application
Recommended Dilution
WB
1:500-1:5000
IF
1:30-1:200
Protocols:
ELISA Protocol Western Blotting (WB) Protocol Immunofluorescence (IF) Protocol
儲存條件:
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期:
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.